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KMID : 0624620080410110803
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BMB Reports
2008 Volume.41 No. 11 p.803 ~ p.807
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Enhanced proliferation of SNU-407 human colon cancer cells by muscarinic acetylcholine receptors
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Park Yang-Seo
Cho Nam-Jeong
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Abstract
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We investigated the expression of muscarinic acetylcholine receptors (mAChRs) and their possible involvement in the regulation of cell proliferation in four colon cancer cell lines (SNU-61, SNU-81, SNU-407, and SNU-1033) derived from Korean colon carcinoma patients. A ligand binding assay showed that all four cell lines expressed mAChRs. Treatment of the four cell lines with the cholinergic agonist carbachol led to the activation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). In SNU-407 cells, carbachol significantly stimulated cell proliferation, which could be abolished by the muscarinic antagonist atropine and the ERK1/2 kinase inhibitor PD98059. These results indicate that mAChRs specifically mediate the proliferation of SNU-407 colon cancer cells via the ERK1/2 pathway.
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KEYWORD
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Cell proliferation, Colon cancer cell line, ERK1/2, Muscarinic acetylcholine receptors
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